Amsacrine(m-AMSA)Description
TopoGENprovidesthisreagentasacontrolpoison/inhibitoroftopoisomeraseII. Forinvitrocleavagedetection,werecommendthatthedrugbetestedoverawiderangeofuMconcentrations;however,cleavagesarestronglyinfluencedbytheamountoftopoisomeraseinthereactionaswellasthetypeofassaybeingemployed(SDSK+vs.RADIoactiveEndLabeledDNAvs.testingforplasmidcleavages). Notethatlargeamountsofenzymewillberequiredtodetectcleavagecomplex,comparedtoassaysthatmeasurecatalyticactivity.
ChemicalName: 4-(9-Acridinylamino)-N-(methanesulfonyl)-m-anisidinehydrochloride
MolecularFormula:C27H29NO10.HCl
MolecularWeight:429.92
VialContents:
TG4150issuppliedalreadysUSPendedinDMSOat10mM(0.25ml)andisreadyforuse. Thedrugispre-testedandshowntoinducetopocleavagesusingpurifiedhumantopoisomerasefromTopoGEN.