TopotecanHydrochloride(Hycamtin)Description
Hycamtin(topotecanhydrochloride)isasemi-syntheticderivativeofcamptothecinandisananti-tumordrugthattargetstopoIandisatopoIpoison(inducescleavagecomplexes).
ChemicalName:(S)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14-(4H,12H)-dionemonohydrochloride
MolecularFormula:C23H23N3O5•HCl
MolecularWeight:457.9
Topotecanhydrochlorideissolubleinwaterandmeltswithdecompositionat213°to218°C. TopoGENprovidesthisreagentasacontrolpoison/inhibitoroftopoisomeraseI. Forinvitrocleavagedetection,werecommendthatthedrugbetestedoverawiderangeofuMconcentrations;however,cleavagesarestronglyinfluencedbytheamountoftopoisomeraseinthereactionaswellasthetypeofassaybeingemployed(SDSK+vs.RADIoactiveEndLabeledDNAvs.testingforplasmidcleavages). Notethatlargeamountsofenzymewillberequiredtodetectcleavagecomplex,comparedtoassaysthatmeasurecatalyticactivity.
VialContents:
TG4100issuppliedalreadysUSPendedinwaterat10mM(0.25ml)andisreadyforuse.Thedrugispre-testedandshowntoinducetopocleavagesusingpurifiedhumantopoisomerasefromTopoGEN.