PolyclonalAntibodytoHumanTopoisomeraseIIIBetaDescription
ArabbitpolyclonalantibodywaspreparedagainstapoolofoligopeptidesderivedfromthecarboxyterminalregionofhumantopoisomeraseIIIb. Thepurityofthepeptideswasgreaterthan95%asdeterminedbyHPLCandmassspectralanalysis. Approximately10mgofpurifiedpeptidewerecoupled(throughaterminalcysteinethiol)toKLHwithaheterobifunctionalcross-linkingagentMBSataratioof1partpeptideto1partKLH. TheantibodytiterwasdeterminedbyELISAtobe>1:10000. ThisantibodyisidealforWesternblottingapplicationsbuthasnotbeentestedforotherapplications. Thedilutionsofantibodyrequiredforspecificdetectionshouldbeoptimizedfortheinvestigator’sparticularsystem(celllinebeingusedforexample).
PolyclonalAntibodyUnitDefinition:
TheantibodyconcentrationisinWesternblottingunits;oneunitcorrespondstoa1:1000dilutionoftheantibodyrequiredtomake1mlofdilutedprobeforaroutineWesternblot.Thus,10unitsofantibodywillmake10mlofdilutedprobe.
AntibodyVialContents:
CatTG2010-4contains250unitsofrabbitantibody (asserum)at2.5units/ul(100ultotalvolume).
MaterialSafetyData:
THISPRODUCEISNOTLICENSEDORAPPROVEDFORADMINISTRATIONTOHUMANSORTOANIMALSOTHERTHANEXPERIMENTALANIMALS.
Theproductissoldasanantibodyforresearchpurposesonly.Safelabpracticesshouldbeemployedwhenhandlingthisproduct.Chemical,physicalandtoxicologicalanalysesoftheproducthavenotbeenperformed.