
PolyclonalAntibodytoYeastTopoisomeraseII(Serum)Description
TopoisomeraseIIwaspurifiedfromSaccharomycescerevisiae. AhomogeneouspreparationoftheenzymewasinjectedintoNewZealandwhiterabbitsandtheanimalswereboostedtwicemore. ELISAtitersweregreaterthan1:105atthetimeofthefinalbleed. TheserawerecollectedandtestedbyprobingWesternblots.Asinglepolypeptidebandof160kDawasdetectedonWesternblotscontainingcrudenuclearextractspreparedfromcellsinmid-logphase. Thisisahighlyspecificantibody,soldascrudeserum(notaffinitypurified)thatwillneutralizeenzymaticactivityofyeasttopoisomeraseII. TheantibodyisspecificforS.cerevisiaeanddoesnotcrossreactonWesternblotswiththehumanorcalfthymusenzymes. Althoughthisreagentmaybesuitableforuseinimmunofluorescentapplications,ithasnotbeencharacterizedforsuchapplications.